BIOB10 Lecture 3.docx

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15 Apr 2012
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BIOB10 Lecture 3
-cell culture: studying cells in a control system. Grow them in a plastic flask in media.
Using cultured cell is good bc you can get them in large quantities, most cultures are
very pure, and a wide variety of cells can be grown
-pluripotent: capacity to differentiate into all possible cell types (reintroduce into
patients where a particular cell is deficient.
-immunofluorescence uses antibodies
ponce protein is fluorescent you can go to either a fluorescent microscope or a
confocal microscope
-part of immune system is B cells (turn on genes to make antibodies)
>antibodies used to tag your proteins.
-antibody genes undergoes nucleotide rearrangements so each b cell has a unique
antibody protein.
-every b cell has a unique gene therefore makes unique IgG and there will recognize
unique foreign antigen
-if you wanna study where actin is in your cell. Need an antibody to this, cant make
it in humans so the human protein is put into a rabbit. There is a b cell in that rabbit
that will bind to actin and make antibodies. Take some blood and then you have
anti-human actin antibodies.
-link fluorochrome to antibody, which will bind to appropriate protein. You have to
fix cells so it can get into cells (immunofluorescence)
--2008 nobel prize for discovery of GFP (extracted from jelly fish and is now used
everywhere)
-Transfect: experimentally introduce DNA into Cell to make protein of interest (i.e
GFP-Protein)
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