BIOB10Y3 Lecture 3: BIOB10 Lecture 3.docx

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Cell culture: studying cells in a control system. Grow them in a plastic flask in media. Using cultured cell is good bc you can get them in large quantities, most cultures are very pure, and a wide variety of cells can be grown. Pluripotent: capacity to differentiate into all possible cell types (reintroduce into patients where a particular cell is deficient. Immunofluorescence uses antibodies ponce protein is fluorescent you can go to either a fluorescent microscope or a confocal microscope. Part of immune system is b cells (turn on genes to make antibodies) Antibody genes undergoes nucleotide rearrangements so each b cell has a unique antibody protein. Every b cell has a unique gene therefore makes unique igg and there will recognize unique foreign antigen. If you wanna study where actin is in your cell. Need an antibody to this, cant make it in humans so the human protein is put into a rabbit.

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