Class Notes for BIOB11H3 at University of Toronto Scarborough (UTSC)

Biob11: lecture 1: chromosomes, heredity, meiosis and recombination. Diploid: containing both members of each pair of homologous chromosomes, as exempl

Lactose = disaccharide of galactose + glucose covalently linked together. Lactose (act as an inducer) -galactosidase expressed lactose metabolized into

Biob11-l16: cell cycle ii (chromosome condensation & movement) Cytoskeleton disassembles; spindle apparatus forms; nuclear membrane breakdown. Chromoso

Sp figures: 12-5, 6, 10, 11, 12, 16. Vocabulary:nuclear pore complex/nuclear matrix/chromatin nuclear envelope/nucleoporins/nuclear localization signal

Biob11-l13: dna replication ii enzymology, origins, organization & licensing: kornberg (1950s) Purified e. coli dna polymerase dna + dntps (datp, dctp*

Biob11-l12: dna replication i general aspects and enzymology. Dna is a double stra(cid:374)ded helix (cid:894)chargaff(cid:859)s rules: [a]=[t], [c]=[g

Biob11-l11: gene regulation 3 epigenetics & miscellaneous gene regulatory mechanisms. Information flow through dna to rna to protein. Mechanisms that a

Translation: association of mrna with ribosomes, and subsequent decoding and formation of a polypeptide chain. Dna mrna proteins: information content:

Mitosis: division of nuclear material // cytokinesis: division of cytoplasm. Consider an asynchronous cell culture (cells in different cell cycle, some

Biob11-l10: gene regulation ii (transcription factors and microarray technology) Transcription factors have two functional domains: dna binding: sequen