BIOB11H3 Lecture Notes - Lecture 10: Leucine Zipper, Zipper, Inverted Repeat
Document Summary
Biob11-l10: gene regulation ii (transcription factors and microarray technology) Transcription factors have two functional domains: dna binding: sequence specific interaction. Often bind to palindromic dna sequences (something that looks like an inverted repeat) May form dimers (homo / hetero) (look for major groove, a wider & deeper than minor groove, has more access to nitrogenous bases in the middle of helix: activation. Must interact with one ore more proteins to promote or repress transcription. Nitrogenous bases have specific functional groups that project into the major groove. Sequence specificity for protein binding resides in the type and order of the bases (functional groups) *many potential of combinations with only 4 bases. Seed specific tf: ry repeats (cat gcat motif) Drosophila bicoid tf: active early in development and help specify different domains and tissues in. Completely different in one strand embryo (not parallel) *the longer the binding site, the more specific it might be (probability of getting all match)
