BIOB11H3 Lecture Notes - Lecture 14: Okazaki Fragments, Dna Clamp, Dna Replication

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18 Mar 2013
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Lecture 14 dna replication ii: enzymology, origins, organization and licensing. Sps: figs 13-12, 13, 14, 15, 19, 20. Interested in dna polymerase and how it works, how many subunits it had, what it did, etc. He purified the protein, he took the dna template, added the raw materials for replication to occur (i. e. deoxynucleotide triphosphates dntp"s) and he radioactively one type of the nucleotides. When the radioactive nucleotide is incorporated into the dna polymer then the polymer will also become radioactive. This is evidence that dna replication is occurring and radioactive materials are being incorporated into the chromosome. Figure 13-7: kornberg was able to purify a variety of different templates like double stranded circles, like single stranded circles, like double stranded dna with partial single stranded character. He discovered that there are two goals for dna replication: 1. Dna polymerase needs a 3" hydroxyl group to get started at a prime site and 2.

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