BIOB11H3 Lecture Notes - Lecture 2: In Situ Hybridization, Hydrogen Bond, Polynucleotide

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turquoisejackal716

14 Oct 2011

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Sps: fig 10-10, 15, 17, 19, 22, 23. Complementary base pairing occurs by hydrogen bonding; a-t and c-g. ! Early work on dna: physiochemical approaches: dna is a polynucleotide chain, estimates made of genome size, values were tiny yet enormous". Humans: 3. 5 picograms of dna/haploid genome = 3. 2 billion base pairs: base pairing rules established by analyzing base composition, dna absorbs light in ultraviolet range, renaturation experiments defined complexity. Ring structures of dna absorb in the ultraviolet range: dna absorption maximum !260nm, absorbance can be used to determine dna concentration. Absorbance increases about 1. 5x if dna is denatured. Thus, changes in absorbance reveal % ds vs. %ss. Consider the satellite dish analogy ssdna; flexible; nitrogenous bases can freely rotate about glycosidic bond dsdna: more rigid, nitrogenous bases limited in # of positions they can occupy. Complexity: a measure of the number of unique (vs. repetitive) sequences that exist in a genome.

Related Questions

Respond to each sentance with true or false regarding the replication of DNA:

Complementary base pairing relies on the number of hydrogen bonds that each base can make.

In a single nucleotide, the phosphate group is attached to the 5' carbon of the sugar unit.

The antiparallel arrangement of double-stranded DNA is due to the phosphate group being bonded to the 3' carbon on one strand and the 5' carbon on the complementary strand.

The phosphate attached to the 5' carbon of a given nucleotide links to the 3' -OH of the adjacent nucleotide.

The -OH group on the 3' carbon of the sugar unit is the attachment site for the nitrogenous base.

rubyclam664

Which of the following are true about nitrogenous bases and base pairing? Select all correct answers.

a. The base composition is such that A equals T and G equals C.

b. The base composition is such that T equals C and A equals G.

c. There are three hydrogen bonds forming the A to T pair and two forming the G to C pair.

d. There are two hydrogen bonds forming the A to T pair and three forming the G to C pair.

e. Bases are stacked 0.34 nm apart.

f. Bases are stacked 0.50 nm apart.

whitezebra886

Place the following steps of DNA replication in right chronological order.
1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides

plumsardine151

BIOB11H3 Lecture Notes - Lecture 2: In Situ Hybridization, Hydrogen Bond, Polynucleotide

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