BIOB11H3 Lecture Notes - Lecture 12: Chromosome, Polymerization, Dna Polymerase Iii Holoenzyme
Document Summary
Lecture 12: dna replication i: general aspects & enzymology. Watson & crick drew double stranded helix that was anti-parallel and each strand was a template for replication of a new strand. *each strand serves as a template for the formation of the complementary strand. Energy & time ef cient; less prone to errors. Used e. coli to investigate the mechanism of replication. Employed a density label: heavy nitrogen (n15) (supplied as 15nh4cl) Grow cells for many generations in n15 medium. Wash cells and switch to normal n14 medium. >upon replication, dna would now have some n15 & n14. After different time points, puri ed dna separated by centrifugation and the n14/n15 ratios are determined. Cscl) and particles sediment until they reach their bouyant density (equilibrium) After one round of replication all molecules sediment at the hybrid (n14/n15) density. After two rounds of replication, half of the molecules are hybrid and half are light (n14/n14)
