BIOB11H3 Lecture Notes - Lecture 10: Expansionism, Nitrogenous Base, Phosphoenolpyruvate Carboxykinase
Document Summary
Lecture 10: gene regulation ii: transcription factors & microarray technology. 5(cid:2) a g a a c a n n n n t g t t c t 3(cid:2) 3(cid:2) t c t t g t n n n n a c a a g a 5(cid:2) Must interact with one or more proteins to promote transcription or repression. *each nitrogenous base of dna have speci c functional groups projected in the major groove. *each functional group interact with side chains of amino acids of transcription factors (proteins) *sequence for protein to bind is based on type & order of bases. Zn coordinates binding of 2 cysteines & 2 histidines. Usually multiple ngers are present, each binding in successive major grooves. 5 zinc nger protein chain (green) on dna. Tfiiia bound to dna of 5s rna gene. Basic domain interacts with dna to determine sequence speci city.
