BIOB11H3 Lecture Notes - Lecture 5: Rna, Rna Interference, Rna Splicing
Document Summary
Spliceosomes are present in higher eukaryotes, mediates rna processing. *snrnas: rna components (snrnp u1 to snrnp u6) 5" splice site of intron, by complementary base pairing on intron. >u2 recruited by u2af thats binded on polypyrimidine on 3" splice site. >u2af also interacts with sr proteins that bind to exonic splicing. >these interactions are vital in recognizing intron/exon borders. >u6 replaces u1 by binding through base pairs. U6, it comes off as u6 binds to intron. >1st chemical reaction to remove intron from 5" splice site. >u5 holds exons together while intron is removed. 2nd chemical reaction ligates exon 1 & exon 2 and excises the rest of the intron simultaneously. >after reaction, snrnas detach and reassociate & reassemble at next intron to be spliced.
