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Lecture 16

BIOB11H3 Lecture Notes - Lecture 16: Maturation Promoting Factor, Microtubule Organizing Center, Cyclin


Department
Biological Sciences
Course Code
BIOB11H3
Professor
Dan Riggs
Lecture
16

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OP16
Cell Cycle II: Chromosome condensation & movement
Smart Pages: Fig 9-11; 14-14, 16, 17, 20, 22, 25, 28, 30
Vocabulary Words: MPF/condensin/lamina/centrosome/centrioles/spindle apparatus/
dynein/kinesin/kinetochore
Central Ideas:
1. Spindle formation and chromosome condensation occur during mitotic prophase.
2. At metaphase, chromosomes are aligned in center of cell, sister chromatids move in
opposite directions at anaphase.
3. At telophase, nuclear membranes begin to form around chromosome bodies, and
cytokinesis is initiated.
4. MPF initiates the G2 to M transition: some of its targets are condensin, lamins, and
microtubule associated proteins.
5. Centrosomes: microtubule organizing center (MTOC); centrioles duplicate during S
phase, move to opposite sides of the cell.
6. A kinetochore is a protein/DNA complex at the centromere of a chromosome and
the structure to which microtubules attach.
7. Molecular motors of both the dynein and kinesin families facilitate chromosome
movement along microtubule tracks.
8. Chromosome segregation is driven primarily by microtubule depolymerization, and
to a lesser extent by molecular motors.
9. Astral, kinetochore, and polar microtubules all play roles in mitosis.
Questions a scientist might ask:
1. How does the phosphorylation of lamins contribute to chromatin condensation and
nuclear membrane dissolution?
2. Between metaphase and telophase, what change would have to occur in the lamin
proteins?
Sample multiple choice question:
Maturation promoting factor:
a. is a complex of a mitotic cyclin and a CDK molecule.
b. promotes the transition from G1 to S phase.
c. phosphorylates lamins A and C.
d. both a and c.
e. all of the above.
Analytical Questions:
1. Questions 1 on pages 560, 562, 567, 571 and questions 9, 13 page 580.
2. If you were to treat a cell with colchicine, which disrupts microtubule
formation, what effect might this have on a cell undergoing mitosis?
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