BIOB11H3 Lecture Notes - Lecture 2: Restriction Fragment Length Polymorphism, Tandem Repeat, Dna Profiling
Document Summary
Detecting satellite dna using fluorescence in situ hybridization. Complexity of eukaryotic genomes (continued: a moderately repeated fraction makes up 20-80% of the genome depending on the organism. It can be repeated a few times to tens of thousands of times and can be coding or non-coding dna: coding dna consists of rrna (ribosomal dna) and histones. Basically coding dna can code for either rna or proteins. Their sequences are usually identical and present in tandem: non-coding dna does not code for any type of product. Its sequences are scattered throughout the genome (interspersed) sines and lines (short interspersed elements and long interspersed elements: a non-repeated fraction are present as a single copy per haploid set of chromosomes. Localization is also highly specific in non-repeated fractions as sequences are present at a particular site on a particular chromosome. Non-repeated fraction sequences code for almost all the proteins except histones: less than 1. 5% of the human genome codes for all our proteins.
