BIOB11H3 Lecture Notes - Lecture 5: Globin, 28S Ribosomal Rna, Ovalbumin

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Sps: fig 11-17,18, 20, 21, 23, 24, 29. For rrna (pol i): initial 45s 28s, 18s, 5. 8s. Pulse chase strategy used (figure 11-17) mrnas are syntheseized as precursors hnrna = heterogeneous nuclear rna. 30,000 active genes many transcripts of various sizes. Most of newly synthesized rna in cell is hnrna but most stable rna of cell is rrna (>90%) Life of hnrna/mrna: minutes to hours (avg 40. Transcription of pol ii gene is regulated by: transcription factors which recruits rna pol, sequences in promoter of gene binding sites for transcription factors and binding sites for pol ii. subunits. Figure 11-20 there is a pre-initiation complex, yellow balls represents the repeats that forms the tail of rna-polymerase. When some phosphate groups are attached then they start to repel form the things binding them. Figure11-21: mrna features, there is a 5" cap,5" untranslated region, coding reasons (triplet sets that protein encodes).

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