BIOB12H3 Lecture Notes - Lecture 6: Agarose Gel Electrophoresis, Restriction Map, Graph Paper
Document Summary
Lab 6: restriction mapping of the unknown plasmid isolated in lab 4 using multiple restriction digests. Part a: set up diagnostic multiple restriction digests. Part b: preparing your samples and loading 1. 2% agarose gel and electrophoresis. Analysis of restriction digests and construction of restriction map of the unknown plasmid using class data. Here are two ways a plasmid can be drawn: as a circle with insert in an arc, where it is difficult to measure distances. 3kb vector: with the insert displayed as a line above the plasmid, so that measurements are readily made. Using graph paper makes it easier to draw it to scale. When restriction mapping the insert it is often acceptable to represent the insert as a straight line with the restriction sites of the mcs surrounding the cloning site and not to represent the plasmid component at all. Restriction mapping is generally used to determine whether sites for particular.
