BIOB10Y3 Lecture Notes - Eukaryotic Initiation Factor, Kozak Consensus Sequence, Start Codon
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Mrnas transcribed from genese by rna poly ii. Exported to cytosol spliced, capped and polyadenylated. 2 subunits (large and small: large = 3 rrnas + proteins, small = 1 rrna + proteins. **imp for exam know difference b/w prokaryotic and eukaryotic subunits ** ribosomes are molecular machines require energy from gtp hydrolysis the ribosomal rnas are key components to their function. Help select the trnas, bind protein factors etc. the crystal structure of the ribosome (prokaryotic) helps us understand how this machine works. A long tunnel runs through the core of the large subunit (50s) and the newly synthesized protein comes through there protected. *we put all 3 types of rna together for translation: trnas (charged with aa) rrnas (ribosomes) Step 1 the small ribosomal subunit binds to the aug (start) codon. In prokaryotes, the correct aug codon is chosen by the presence of an upstream sequence: the shine-