BIOB12H3 Lecture Notes - Lecture 2: Spectrophotometry, Pipette, P200
Document Summary
Use of pipettes and micropipettes, spectrophotometry, quantification of dna and protein and bacterial media preparation: measuring liquids, spectrophotometry, protein quantification, dna quantification, data analysis/class discussion of spectrophotometry exercise, making solutions: bacterial media preparation for week 3 laboratory. Outline of lab 2a (first lab of the week) Outline of lab 2b (second lab of the week) on bacterial growth. In a biology laboratory, it is common place to measure liquids. The volumes needed can range from liter to ml to microliters. Graduated cylinders are used for volumes from approximately 10 ml to a liter quantity. Glass pipets are used for volumes from 0. 1 ml to 10 ml. For volumes below one ml one can use micropipettes. For volumes below 0. 1ml a micropipettors is an absolute requirement for accurate measurement. The results of your experiment will depend on the art of measuring liquids. It is a learned skill that takes practice.
