BIOC12H3 Lecture Notes - Lecture 9: Catalytic Triad, Valine, Ionic Bonding
23 Sep 2016
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Slide 8: graph a. x axis a. i. course of reaction a. ii. reaction coordinate a. iii. progress of bond breaking proceeds to the right, y axis b. i. free energy, do not need to know how to draw enzyme catalysis diagrams. Slide 27: know when its active and inactive and why. Slide 30: know the steps, know what happens, visualize it and understand the steps, point form, mechanism a. step 1 a. i. Dhap binds to enzyme a. ii. carbonyl of dhap forms h bond with his a. iii. carboxylate of glutamate is ionized (negative) takes proton away from c1 b. step 2 b. i. Slide 39 a. i. binding site channel is lined with hydrophilic residues a. i. 1. why not hydrophobic? a. i. 1. a. because the free oxygen radical is negatively charged, the binding site needs to be hydrophilic a. i. 2. Missed what she said about positive charges a. i. 3. What emphasis? a. copper important because it is positively charged in superoxide dismutase a. i. also important in shielding negative charges? a. i. 1.
