BIOC12H3 Lecture Notes - Lecture 4: Protein Sequencing, Primary And Secondary Antibodies, Protein Structure
19 Jul 2020
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Lecture 4 -- protein sequencing, prufication, and related methods. Western blotting: conducted in order to confirm the presence of a specific protein in a protein population. Not shown (but implied) is taking the gel and actually running it. In green, we see the secondary antibody attached to the constant region (add a tag) We must block all the non- specific sites (block places where the antibody can stick. Variable region interacts with the antigen, constant region is sticking out. The original polypeptide has 2 peptide bonds (or. Hcl at a high temperature and concentration: the three amino acids can then be determined via percentage composition within the actual protein using analytical techniques. Semester: summer 2020: amino acid hydrolysate treated with phenylisothiocyanate (pitc) Pitc reacts with the amino acid to give us a detectable fragment under the hplc. Not perfect, but does the job for the most part!
