BIOC15H3 Lecture Notes - Lecture 9: The Sequence, Genomics, Plasmid
9 Mar 2021
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Document Summary
6-fold, 3-fold, and one-fold coverage, respectively: the "paired-end sequencing" strategy overcomes the problem posed by identical repetitive dna sequences scattered throughout the genome, finally, a genome-wide shotgun computer program assembles all these sequences into chromosomal strings. Whole-genome shotgun sequencing strategy: only three libraries of differing-sized fragments (2 kb, 10 kb, and 200 kb) need to be constructed, the challenge is to assemble these sequences when they include large numbers of repeats. The cost of genome sequencing: cost is indicated by the plot in green, the pace of moore"s law (the observation that computing power doubles every 18 months) is shown in white. Alternative splicing: collectively the alternate promoter and alternate splicing can allow production of about. 1,000 different mature mrnas from this one gene. Vdj recombination: by allowing any possible v d j joining, from this one region of the human genome, nearly 1000 different immune proteins can be made.
