BIOC17H3 Lecture Notes - Lecture 8: Gram Staining, Lipid Bilayer, Peptidoglycan

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10 Aug 2019

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Gram staining allows us to know the difference between gram negative and gram positive. The main function of cell wall is to resist hydrostatic pressure that builds u in the cell. The cytoplasm contains many proteins, ions and molecules, the bacterial cell is found in hypertonic environment. So, there is more solute inside the cell to the outside. Water will low into he cells due to the osmosis. Remember it goes form low to high concentration. The bacterial cell will have increase hydrostatic pressure because it will have lots of water in it. If they did not have this cell wall and more water keeps coming into the cell, then it will burst. The cell wall of bacterial cell withstands hydrostatic pressure inside the cell. The cell wall is made up of peptidoglycan which is a sugar molecule with peptides. Each disaccharide has many amino acids which is connected to an adjacent sugar.

Related Questions

1,Match the descriptions to the type of transport.

- A. B. C.	Water enters a cell because it is hypertonic compared to the extracellular environment, increasing its turgor pressure
- A. B. C.	Water enters guard cells because the guard cells are hypertonic compared to the surrounding cells
- A. B. C.	Potassium ions are pumped into guard cells
- A. B. C.	The motor cells of a Venus fly trap are hypotonic compared to surrounding cells, causing water to leave the motor cells
- A. B. C.	Sugar is loaded into the phloem

A.	Both active and passive transport
B.	Passive Transport
C.	Active transport

2,Describe what is wrong with the following statement and explain why:

All prokaryotic and eukaryotic cells contain plasma membrane, nucleus, ribosomes, organelles, cytoplasm, and cell walls.

What is short-distance transport?

	a,	The movement of solutes from a region of low concentration to a region of high concentration
		b,The movement of water, nutrients, and other substances between cells
c,		The movement of substances from a region of high concentration to a region of low concentration
d,		The movement of water across a semipermeable membrane from a region of low solute concentration to a region of high solute concentration.

What terms describe the movement of water across a semipermeable membrane from a region of high water concentration towards a region of low water concentration (and high solute concentration.)

What is found in the proptoplasm?

Cell wall, cytoplasm, plasma membrane, nucleus, and other cell organelles

Plasmodesmata, cell wall, and plasma membrane

Cell wall, cytoplasm, and plasma membrane

Cytoplasm, nucleus, and other cell organelles

The channels that allow nutrients and protoplasm to pass between plant cells are called _________________.

What happens when a plant cell is placed in a hypotonic solution?

Water will enter the plant cell and the plasma membrane will swell and cell lysis will occur

Water will leave the plant cell and the plasma membrane will pull away from the cell and plasmolysis will occur

Water will enter the plant cell and the plasma membrane will swell and push against the cell wall

Water will leave the plant cell and the plant cell will lose turgor pressure, causing the plant to wilt

________________ transport uses cellular energy.

What molecules make plasma cell membranes hydrophilic on the outside and hydrophobic on the inside?

What type of cells do plants have?

		Bacterial
		Archaeic
		Eukaryotic
		Prokaryotic

HELP WITH BIO MULTI CHOICE QUESTIONS:

QUESTION 2

Which of the following structures discussed in class is NOT found on all bacteria?
Flagella
Capsid
Fimbria
Plasma membrane

QUESTION 3
You are presented with a strain of bacteria that is resistant to the antibiotic penicillin. What steps would you take to confirm that you have a resistant bacterium?
Perform a Gram stain to determine pink stained Gram-positive bacteria.
Perform a Gram stain to determine purple stained Gram-positive bacteria.
Perform a Gram stain to determine pink stained Gram-negative bacteria.
Perform a Gram stain to determine purple stained Gram-negative bacteria.

QUESTION 4
With respect to bacterial growth into a fresh culture tube containing nutrient broth, at which phase do organisms undergo binary fission the most rapidly?
Lag phase
Stationary
Exponential
death

QUESTION 5
How would antibiotic resistance be passed from one bacterium to another bacterium?
Through the flagella via a plasmid.
Through the sex pilus via chromosomal DNA.
Through the sex pilus via a plasmid.
Through the flagella via chromosomal DNA.

QUESTION 6
When an F plasmid integrates into the host chromosome, the strain is referred to as

Hfr.
F'.
F-.
F+.

QUESTION 7
If you remove the peptidoglycan layer from a Gram-positive cell, it would still stain purple with a Gram stain (T/F).
True
False

QUESTION 8
During infection with a bacteriophage, bacterial DNA is packaged into a capsid and will:

be integrated into the genome of a new bacterium upon ligation.
be integrated into the genome of a new virion upon recombination.
be integrated into the genome of a new bacterium upon recombination.
be integrated into the genome of a new virion upon liagation.

QUESTION 9
Which of the following statements best describes most bacteria?
They are generally harmful.
They are limited to living in a few habitats.
They are very common in the environment.
They are responsible for the âcommon cold.â

QUESTION 10
Prokaryotes lack some parts found in eukaryotic cells, including which item?
a nuclear membrane
DNA
one or more chromosomes
a plasma membrane
all of the above

QUESTION 11
With respect to gram negative bacteria, what is the order of the bacterial cell structure from outside to inside?
Thin peptidoglycan layer, plasma membrane

thick peptidoglycan layer, plasma membrane

outer membrane, thick peptidoglycan layer, plasma membrane

outer membrane, thin peptidoglycan layer, plasma membrane

QUESTION 12
In transformation, a piece of DNA from a dead host can be taken up by living host

True
False

QUESTION 1

Which of the following statements about the bacterial cytoplasmic membrane is false?

	a.	It resists attachment of the bacterial chromosome to its inner surface.
	b.	It contains a protein complex that drives ATP synthesis.
	c.	It is asymmetric.
	d.	It consists of relatively equal amounts of phospholipids and proteins.

1 points

QUESTION 2

Which of the following statements regarding Mycobacteria is false?

	a.	The genome has relatively low Guanine (G) and Cytosine (C) content.
	b.	The cytoplasmic membrane is surrounded by a polymer (peptidoglycan) consisting of alternating N-Acetylglucosamine (NAG) and N-Acetylmuramic (NAM) acid units.
	c.	It infects approximately one-third of the global human population.
	d.	It has a Gram-positive cell wall with an additional waxy lipid layer of mycolic acid.

1 points

QUESTION 3

Which of the following outer membrane porins enables Mycobacteria to adapt to acidic and intracellular environments, and shows homology to outer membrane porin A?

	a.	OmpATb.
	b.	OmpA.
	c.	MspA.
	d.	Lipid A.

1 points

QUESTION 4

Based on your understanding of bacterial membranes, which of the following is not likely to be found in an E. coli outer membrane?

	a.	Membrane-anchored enzymes.
	b.	Mycolic acid.
	c.	Lipopolysaccharide.
	d.	Porin channels.

1 points

QUESTION 5

Based on the conclusions of this paper, which of the following statements would the author most likely disagree with?

	a.	Some archaeon do not contain all of the genes essential for life.
	b.	The membrane structures of prokaryotes show far greater diversity than those of eukaryotes.
	c.	As demonstrated in this definitive report, scientists have a great understanding of the majority of basic functions and evolution of bacterial membranes.
	d.	The outer membrane (and its associated components) of archaea, Gram-negative bacteria, and Gram-positive bacteria likely evolved independently of one another.

1 points

QUESTION 6

Which of the following is not true for Gram-negative, acid-fast, and archaeal membranes?

	a.	They modify their lipid content in response to environmental changes.
	b.	They have co-evolved the same outer membrane composition and structure.
	c.	They all have a cytoplasmic membrane.
	d.	Membrane transport proteins regulate nutrient uptake and waste removal.

1 points

QUESTION 7

Compared to Mycobacteria, Gram-negative bacterium contain:

	a.	More mycolic acid.
	b.	Pores of increased length.
	c.	Less peptidoglycan.
	d.	50-fold-lower numbers of porins.

1 points

QUESTION 8

Archaea are found to survive in strongly acidic lakes compared to bacteria and Eukaryotes. Which of the following supports this claim according to the study?

	a.	The presence of L-glycerol ether linked lipids in Archaea membranes.
	b.	The presence of D-glycerol ether linked lipids in Archaea membranes.
	c.	The presence of L-glycerol ester linked peptidoglycans in Archaea membranes.
	d.	The presence of L-glycerol ester linked lipids in Archaea membranes.

1 points

QUESTION 9

Based on your understanding of the symbiotic relationship between Nanoarchaeum equitans and Ignicoccus, which of the following below is not true?

	a.	The membrane lipids of N. equitans are synthesized by Ignoccocus.
	b.	In exchange for membrane lipids, N. equitans provide Ignicoccus with many essential cytoplasmic enzymes.
	c.	N. equitans lack enzymes capable of catalyzing lipid biosynthesis.
	d.	Intraperiplasmic vesicles produced by Ignicoccus allow for lipid transfer to N. equitans.

1 points

QUESTION 10

What is the role of hydrogen bonds in bacteria, as mentioned in the article?

	a.	They mediate transmembrane electron flow.
	b.	They mediate lipid biosynthesis.
	c.	They help to stabilize the cytoplasmic membrane.
	d.	They are responsible for the selective permeability of the cytoplasmic membrane.

BIOC17H3 Lecture Notes - Lecture 8: Gram Staining, Lipid Bilayer, Peptidoglycan

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