BIOC15H3 Lecture Notes - Lecture 5: Epistasis

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We also now know how to do genetic dissection to learn more about the genes phenotypes determining traits: important technique, bittle and tatum, looked at the biochemical process by which neurospora can make arginine. We looked how multiple genes can affect one phenotype, but only looked at homozygous mutat phenotypes and the phenotypes of the f1 heterozygous: ex: 4 genes contributing in a biochemical pathway to the final production of arginine, we have only looked at true breeding lines and heterozygotes, heterozygotes gave rise to heterozygote complementation test. Monohybird (4 boxes: aa x aa p, aa x f, if dom/ rec 3:1, 1 aa: 2aa: 1aa. Dihybrid cross involving complementary gene action: heterogeneous traits and complementation, it had white flower for a different reason, you need dominant allele of both genes to make the purple, f2: 9 purple: 7 (=3+ 3+ 1)white phenotypes, f2 genotype.

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