Class Notes (806,814)
Canada (492,451)
BIOB10H3 (234)
Lecture 3

BIOB10 - Lecture 3.docx

4 Pages
Unlock Document

University of Toronto Scarborough
Biological Sciences
Rene Harrison

Lecture 3 BIOB10 Lecture 3 Cell Culture Microscopy 1. 2. Cell Biology Techniqus: Cell Culture  Cell culture: o Studying cells in controlled, in vitro system o Grow in plastic flasks in media  Aqueous solution used to give them nutrients  Why use cultured cells in research? o Cultured cells can be obtained in large quantity o Most cultures contain only a single type of cell o Wide variety of different cells can be grown in culture o Minimized direct animal research but def is indirectly animal research  Just take muscle cells and grow them indefinatley but do not need to actually use/kill animals for this study 3. Image 4. HeLa Cells  Immortal cells that are used in a lot of lab experiments  From a woman who died from cervical cancer in 1951 and the family does not have consent over the uses of it 5. Cell Culture  Why use cultured cells in research o Many different cellular activities can be studied o Chance to study cell differentiation o Cultured cells respond to treatment with drugs, hormones, growth factors and other active substances o Stem Cell Therapy  pluripotent (capacity to differentiate into virtually every other cell type) o To Treat: juvenile diabetes, Alzheimer’s , Parkinson’s, spinal cord injuries, etc. 6. Cloning = Somatic Cell Nuclear Transfer  LOOK AT SLIDE  take a somatic cell from a patient  take nucleus out of it to obtain identical genome and proteins as patient  transfer it to an empty egg (enucleacted oocyte)  stimulate development of the egg to the blastocyst stage which is when embryonic stem cells grow (ES cells) o a problem with this technique is that the proteins end up being the age of the nucleus when it was removed Lecture 3 BIOB10  At this stage they should be harvested and expose them to factors to make them into certain cells  Introduce cells back into the patient and not have rejection since the nucleus’ match up  Induced pluripotent stem cells (iPS)  2012 Nobel Prize 7. The Sizes of Cells and Their Components  8. Bright-field Light Microscope  Uses a light that focuses on the specimen  Visualizing cells: o Fix cells: adding a fixadive and killing the cell o Stain cells: use a chemical stain; non-specific o Mount on slides  Tissues: embed and section first 9. Using Bright-Field/Light Microscopy  H and E (stain) of sections from gene knock-out mouse  Stain can highlight areas of defect 10.Other Light Microscopes  Phase contrast and Differential Interferences Contrast (DIC) o Form small, unstained specimens like living cells  Want to increase contrast without staining  Used routinely  Use specialized optics to increase contrast 11.Light Microscopes  Useful in examination of intracellular components in cells (fixed or living) at rel
More Less

Related notes for BIOB10H3

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.