Class Notes (809,264)
Canada (493,601)
BIOB10H3 (234)
Lecture 9

Lecture 9

7 Pages
Unlock Document

University of Toronto Scarborough
Biological Sciences
Rene Harrison

Lecture9110811Studying Proteins Osteoclasts and osteoblastsparticularly with astronautseOSTEO hardwarecell culture system for spaceFoton and SoyuzEffects of microgravity on osteoclast bone resorptionStudying Proteins A Determining Primary structure 1 Extrapolate from genomic sequence 2 Manually sequence the primary structure REQUIRES PROTEIN PURIFICATION Genomic Sequence y Extrapolate amino acid sequence from genenucleotide sequence using computer databases of genomes y 1980s and 1990sage of genomics y The Human Genome Project vs Celera Cragi Ventorfound Celera Genomics in y 1999 DNA sequence snow in Genback y 1 Extrapolating Genomic Sequence This has 2 limitationsissues I Intronsexonsexons code for proteins introns intervening sequences dont code for anythingSPLICED OUT OF mRNA will not correctly predict protein II Posttranslational modifications often cannot be predicted from genomeIE PROTEOLYSIS AND GLYCOSYLATION 2 Manually Sequence the Primary Structure The age of proteomicssequencing the y Mass Spectrometry primary sequence of proteins y FIRSTPURIFY PROTEINS a Proteins either separated from complex protein mixture on gel b Or proteins purified using chromatography Purifying Proteinsa Protein Gel Electrophoresis
More Less

Related notes for BIOB10H3

Log In


Don't have an account?

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.