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Lecture 6

Molecular Cell Biology Lecture 6.docx

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Biological Sciences
Dan Riggs

Molecular Cell Biology Lecture 6 1. Conserved (consensus) sequences exist at the junctions of introns/exons 2. These sequences are recognized by spliceosome components and interactions occur 
 by RNA::RNA base pairing. 3. Self-splicing RNAs exist in nature and can be categorized as group I or group II 
 introns. This involves elaborate intrastrand basepairing, forming complex 3D structures. Self-splicing implies that RNA has catalytic activity, like an enzyme-thus the term „ribozyme‟ was coined. 4. Northern blotting (RNA gel blotting) can be used to demonstrate the processivity of RNA splicing as well as alternative splicing (we will get to this as a means of gene regulation in chapter 12. 5. The consequences of incorrect splicing: frameshifts and the generation of missense and/or nonsense mutations, abolishing protein function. 6. Small interfering RNAs (siRNAs) and microRNAs (miRNAs) play roles in development and defense by mediating destruction of dsRNA or inhibiting translation. o How does the same basic splicing machinery recognize the exon-intron boundaries in thousands of different pre-mRNAs?  The sequence most commonly found at the exon-intron borders within mammalian pre-mRNA molecules is shown in Figure 11.30  The G/GU at the 5’ end of the intro (5’ splice site), the AG/G at the 3’ end of the intron (3’ splice site), and the polypyrimidine tract near the 3’ splice site are present in the vast majority of eukaryotic pre-mRNAs  In addition the adjacent regions of the intro contain preferred nucleotides, which play an important role in splice site recognition  Additional clues that allow the splicing machinery to distinguish between exons and introns are provided by specific sequences, mo
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