BIOB11H3 Lecture Notes - Lecture 10: Phosphoenolpyruvate Carboxykinase, Zinc Finger, Dna Microarray
Document Summary
Lecture 10 gene regulation ii: transcription factors and microarray technology. Steps: in a), each well on the plate holds a small volume containing a specific, cloned yeast gene. Dnas that represent the mrnas present in a cell) (step 2). cdnas are prepared from 2 different cell populations, one labeled with a fluorescent green dye and the other with a fluorescent red dye. Any spot in the microarray that exhibits fluorescence represents a gene that has been transcribed in the cells being studied. The existence of several families of dna-binding proteins indicates that evolution has found a number of different solutions to the problem of constructing polypeptides that can bind to the. Dna double helix: most of the motifs contain a segment (alpha helix) that is inserted into the major groove of the dna, where it recognizes the sequence of base pairs that line the groove.
