BIOB11H3 Lecture Notes - Lecture 12: Nucleoside Triphosphate, Dna Gyrase, Prokaryote

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28 Feb 2018
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Then the old fragments and new segments would be joined together to form a complete strand. The daughter duplexes would contain strands that were composites of old and new dna. This model came up to avoid the immposible task of unwinding 2 interwined strands of a dna duplex as it replicated. 13. 3 experiment demonstrating that dna replication in bacteria is semiconservative. 13. 4 experimental demonstration that dna replication occurs semiconservatively in eukaryotic cells: mammalian cells were allowed to undergo replication in brdu, a compound that is incorporated into dna in place of thymidine. Brdu-containing strands, whereas the other chromatid was a hybrid consisting of a brdu- containing strand and a thymidine-containing strand. The thymidine containing strand had been part of the original parental dna molecule prior to addition of brdu to the culture. 13. 6 the unwinding problem: separation of the strands at one end would be accompanied by rotation of the entire fiber as it resisted the development of tension.

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