BIOB11H3 Lecture Notes - Lecture 6: Rna Interference, Intron, Missense Mutation
Document Summary
Lecture 6: rna splicing, catalytic rnas and rna interference part 1. Sp 11. 30- nucleotide sequences at splice sites of pre-mrnas. A pre-mrna contains info that directs the machinery responsible for rna splicing. Dna is removed during the process of protein creation: accurate splicing removes intron, so adjacent exons are joined to form a contiguous coding sequence. When translated, makes aberrant protein (has xtra amino acids- changes structure, and potentially it"s function): triplet set of nucleotides encode each of these xtra amino acids, missense protei(cid:374) produ(cid:272)ed (cid:374)or(cid:373)al fu(cid:374)(cid:272)tio(cid:374) of protei(cid:374) (cid:373)ay (cid:271)e lost! Example: 1st sequence shows normal splicing of pre-mrna (intron in red completely taken out). 2nd sequence is mis-sense protein fu(cid:374)(cid:272)tio(cid:374) of this protein may be lost. Sp 11. 31- the structure and self-splicing pathway of group 2 introns (a) 2d structure of a group ii intron (shown in red). (the intron folds into six characteristic domains that radiate from a central structure).
