BIOB11H3 Lecture Notes - Lecture 3: Transposase, Transposable Element
Document Summary
Lecture 3 genome evolution, gene mapping and. Individual colourless kernel if transportation occurs late in development get small spot of pigment. If transposition occurs early: large coloured sector, due to transposon jump early in development. You take recipient and repeat, insert transposable elements that after transposition you have duplicates. Generation of direct repeat: target/recipient site, staggered cuts are made (transposase, end structure, transposon inserts, repair synthesis generate direct repeat. Other picture is cut/paste method of bacterial transposition. Dna, transposase binding, then cleavage to target dna, integration occurs and a results in strip with donor-dna-transposon. It"s non replicative, replicative involves rna intermediates called retrotransposons. Genetics approach: examine recombination frequency b/w 2 traits, usually visible, to estimate distance b/w 20linked genes. Distance b/w genes on x-some proportional to probability that recombination will occur b/w them. Centimorgan: map unit of recombination; 1cm=1% recombination b/w 2 linked genes. Use crude" information from genetic map general order" and linkage of genes.
