BIOB12H3 Lecture Notes - Bio-Rad Laboratories, Asepsis, Pasteur Pipette

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9 Apr 2014
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Biob12h3s: cell & molecular biology laboratory. The macromolecules include proteins, nucleic acids, sugars, fats and other cellular components. Once the cells are broken open, the resulting suspension is termed a homogenate. If you are only interested in looking at the total protein population by. Once the cells are broken open, in many cases subcellular fractionation is undertaken. This usually centers on differential centrifugation to sediment subcellular components that differ in their size and density. Centrifugation generates a liquid fraction (the supernatant), and a solid or particulate fraction (the pellet). Subcellular fractionation is always coupled with either a marker enzyme assay and/or a functional assay for the molecule of interest. The functional assay is generally devised by the. 1 investigator to screen for the presence of the component of interest. Obviously, in order to devise a test, one must know something about the properties and/or function of the molecule.

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