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Lecture 2

Lecture 2 on Resting Membrane Potential

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University of Toronto Scarborough
Biological Sciences

BIOB30Fall2011 Chapter 5 Membrane DynamicsLecture 2 The Resting Membrane Potentialo There is as typical concentration of ions such as K and Na in intracellular and extracellular solutionsthis creates a concentrationchemical gradient between the IC and EC compartmentso The chemical gradient between the IC and EC exists because of different properties of the plasma membrane which separates them o The membrane is relatively impermeableonly certain molecules diffuse through easily o Water and small non polar molecules diffuse through easilyo Large molecules such as glucose diffuse very slowly o Polar molecules other than water cannot diffuse throughthe plasma membrane is impermeable to them o The reason for the chemical gradient is the NaK ATPase pump located on the cell membrane o The pump transports 3 Na ions out of the cell and simultaneously transports 2 K ions into the cell o The pump is referred to as an electrogenic pump because it produces a net movement of positive charge outside the celltherefore the inside of the cell is negative relative to the outside of the cell o The pump moves ions against their chemical gradienttherefore it requires energy from ATPactive transport because it requires energy o To get this energy ATP must be hydrolysed to ADPo The inside of the cell is high in Na and the outside of the cell is high in K o Electrophysiology is used to measure the membrane potential difference Vm of living cells o A recording electrode is placed inside the cell and a ground reference electrode is placed in the the salt bath the cell is placed in and given a value of 0 mV o The voltmeter measures the difference in electrical charge between the inside of the cell an
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