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Lecture 11

Lecture 11

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Biological Sciences
Patrick Mc Gowan

Lecture 11 y Article on Behavioural phenotyping strategy for mutant mice o McGowans lab interested in stress and genes involved with stress y Crowley Expert in phenotyping analysis She talks about the the way you can use behavioural assays to get questions about whether genes are involved in particular behaviour traits Maybe these behavioural traits are related tohave predictive values for mental disorders in humans y Its important to understand that were not trying to say that a mouse is depressed we can never say that they are depressed or schizophrenic but we can say that the mouse models depression This means that the mouse presents certain behavioural phenotypes that are reminiscent to symptoms of the disorders in humans or they are responsive to drugs that are affected in humans for treating these symptoms of diseases This is a general framework of understanding how we use behaviours in animals to study symptoms of mental diseases in humans Phenotypic Analysis of Animals Behaviourdifferent ways of studying behaviours in animals All these teststasks are ones that are very commonly used in laboratories and they have a very good ability to discriminate differences among different strains of mouse But they may be better or not better depending on strain differences in behaviour or because of the genes that are knocked out that may be responsive to some of the assays or may not be Lot of work has gone into trying to understand behaviour in animals when we use these studies to analyse knockout mice some of the tasks below work better than othersLearning and Memory Spatial Navigation y Morris Water Maze the task involves having a pool of water which is opaque by putting in paint and it relies on the difference in contrast between the pool and the animals The animal is supposed to figure out where it is depending on the various cues that are placed around the pool There is also a platform underwater which will allow the mouse to escape from the pool If the animal swims around enough and bumps into the platform it will be able to sit there And if it sits there for 30 sec the experimenter picks it up and the mouse escapesy Idea The idea is that the mouse is placed in the water it will swim around madly And it will bump into the platform and over a number of times of doing that the animal learns where the platform is unless there is a brain damage Then you can see the path it takes trajectoryhow quickly it finds the platform how they find the platform over repeated trials as an index of learning spatial memory y There are other tasks and all of them use enforcement escapethe mouse wants to escape y Barnes Maze Morris water maze without the water It is a round circular area with lots of holes in it one of them has an escape hatch and the mice need to find the one hole where the escape hatch it Again the mice need to use the cues to find its way You can tell that they are using those
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