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Lecture 2

Lecture 2 Annotated

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University of Toronto Scarborough
Biological Sciences
Shelley A.Brunt

Slide 1Lecture 2 microbe identification and diversityRelated chapters 1 3 of custom text732014Brunt 20141 Slide 2Kochs18431910 PostulatesA set of commonly accepted criteriawhich need to befulfilled to demonstrate that a particular organism is the likely cause of a particular diseaseOriginallywritten for diseases of animalsStill are thestandards for epidemiology the study of the causes of disease or injuryPostulatesRule 1 the causative organism should be found in animals suffering from the diseaseand should not be found in healthy individualRule 2 The organismmustbe isolated and cultured in pure culture away from the diseased animals bodyRule 3Such a cultureorganisms when inoculated into a susceptible animalshould cause the characteristic disease symptoms in the second hostRule 4the organism should bereisolatedfrom these experimental animals culturedagain in the laboratory and should be thesame as the organism isolated from the original host732014Brunt 20142These are the rulespostulates and standards for epidemiology the study of causes of diseases or injury Rule 1 The opportunistic pathogens would be found in healthy individiuals and thats a small subset of organisms Generally if you have measels you can find the evidence of virus in you but you cant find it in someone who is healthy Rule 2 The world of bacteria and growing them in media Theoritcally virus are culturable but you need to put them in a specific medium Rule 3 You can produce the same effects from one host to the next Rule 4 The organism from the second susceptible host shuold be the same as the orginal from the original host This is limited to animal modelsSlide 3streaking for isolated colonies of Serratiamarcescens732014Brunt 20143Petridish with bacteria You need to have a pure culture with individual colonies This is a mesophile and a pathogen grown at 37 degrees Streaking is to produce a pure culture where one colony does not touch another colony
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