BIOC17H3 Lecture Notes - Growth Medium, Ligier Js1, Microbiological Culture
22 Jul 2014
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Immediately aseptically remove 5ml of bacterial culture and place into spec20 tube for turbidity reading. Tell ta turbidity reading as soon as you obtain it: remove 0. 1ml from culture flask for the zero time viable cell count. 37 or 30 degrees in the shaking water bath. Turbidity steps: first zero the spec 20 using left knob. This removes fingerprints that may alter light scattering: repeat turbidity readings every 15 minutes (0, 15, 30, 45 60, 75, 90, 105, 120). After removing the sample, immediately return to the flask to your assigned temperature: remove the bacterial sample from culture flask using aseptic technique, does not have to be kept sterile. However, the 5ml for the blank must remain free of bacteria: record these turbidity readings in a table, hand in a copy of these readings to ta, on semilog paper, plot turbidity versus time for both growth temperatures. From this you can read generation time off the graph.
