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Lecture 05

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University of Toronto Scarborough
Biological Sciences
Ian Brown

REVIEWCLARIFICATIONTranscription factors that can turn on selective genes transacting proteins and transcription factors are the same thingFrom the red blood cell lecture signal elements are sequences in promoter sequencesLens lectureCell peeling is used to isolate lens cells at different stages of development You take an isolated lens and put it in a medium that does not have magnesium or calcium in the mediumCells that have receptors this medium will allow these layers to come off so the cell will not stick to the surfaceThis will cause the layers to peel offThe first layer is the epithelial layerThen we pull the lens out of the medium and in the beaker the first layer will remainPlace the lens back into the medium to strip off the next layerThis is how the lens is preparedIt is like peeling the layers of an onionWe then have the three 3 stages of cell differentiationWe use the separation technique DEAcellulose under low salt to increasing salt solutionThis fractionates the three 3 types of crystallineWe can then look at the amount of protein and crystallin present in the different layersLECTURERole of hormones and developmentWe have looked at the differentiation of a specific cell type red blood cells Tissues are also differentiating at the same timeHow is the difference in cell types coordinated at the same timeHormones play a big roll in this coordination process to make all the components of the developing animalExampleAn example would be frogs and insects that undergo metamorphosis when they transform from juvenile to the adult formThe frog will transform from a tadpole to a frogThe insect will transform from the larval to the adult stageIn both the frog and the insect specific hormones regulate and coordinate these eventsThe metamorphosis hormone is called thyroxineIt controls the change from a tadpole to a frog ecdysone in insectsInsectsPage 1
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