BIOC23H3 Lecture : Tandem Mass Spec (word-for-word lecture notes)

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Determine molecular weight: 2) mass spec fingerprinting; after trypsin digestion can detect set of peptides in mass spec and search database to determine what exactly the protein is. Determine what protein: 3) tandem mass spec: to determine peptide sequence; tandem means 2 mass analyzers/filters/detectors used side by side and pick one peptide and then determine peptide sequence. Slide 2: tandem mass spec instrument; how peptide sequence works. Slide 4: from the first mass analyzer, choose a particular peptide as the one shown with 10. Slide 5: another method for protein sequencing = edman degradation. Aa sequence have sequence and search database to determine protein. Like first lab = mitochondrial protein isolation; this technique will demonstrate all the proteins localized in the mito: f. Slide 10: mudpit is also 2d, when we talk about the classical 2d gel, it"s a combo of isoelectric focusing and.

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