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Lecture 5

BIOD29 Lecture 5 Monday January 20.doc

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Department
Biological Sciences
Course
BIOD29H3
Professor
Aarti Ashok
Semester
Winter

Description
BIOD29 Lecture 5                      Monday January 20,  2014  Matsuyama et. al, 2005 □ Immediate implications   Matsuyama et. al, 2005 pt. 2 □ Linking to literature  SARS: Lessons Learned □ 5 million people in the city in the city, during the SARS outbreak, no problem  with people turning themselves in with early state symptoms □ Did good in being able to put in public health measures to avoid viral spread □ ? ­ Had to enforce a law for quarantine  Larger Implications of this Work  Middle Eastern Respiratory Syndrome Coronavirus (MERS­CoV) □ So far not in Canada □ Total fatality rate approaches 50% (slightly higher than SARS) □ Not yet convinced with human to human transmission □ Ongoing epidemic  □ Cases documented in UK, North Africa and France □ Thought to have started in Jordan  Saudi Arabia □ Correlation at pathogen, coronavirus level and at the point of cell entry  □ Can our understanding of SARS­CoV and the cell biology of its infection,  help us combat MERS­CoV Class 3A Bafilomycin – blocks endosomal pathway  protein found on the surface of endosomes (vacuolar ATPase; allows protons to be pumped into the interior to maintain low pH)  Baf attaches itself to the pump so it does not pump in proton  Mononegavirales □ All 3 families of viruses share a lot of replication strategies, share order of  proteins present in their genome  Mononegavirales pt. 2 □ Bridging the nucleopcapsid protein out to the spike proteins on the envelope □ Few molecules –will synthesize more as it transcribes its genome  comes  prepackaged with RNA­dependent RNA polymerase (protein L) □ Makes 5’ to 3’ copy of the genome □ Can sequentially transcribe 5­10 genes, although it is really good at making  one or two proteins and falling off, then coming back to transcribe more  Rhabdoviridae □ Rhabdo – bullet shape (seen budding form infected cell) □ Spring  covered by piece of cell membrane form the infected cell that  causes the bullet shape  Rhabdoviridae: Rabies Virus □ Has a broad tropism □ Rabies – prototypic virus  P is a co­factor for L protein □ M protein is coding nucleopcapsid □ Also present it’s the L and P proteins □ P  phosphoprotein □ RNA­dependent RNA polymerase is not sufficient, brings a ‘friend” to telling  it to switch from translation to …15:00  Paramyxoviridae □ Not the same as coronavirus which has trimer spikes □ Bind mucalproteins on our cell surface  Paramyxoviridae: Measles Virus □ Fusion is at the cell surface (different from rhabdo – fuses with endosomal  membranes)  Paramyxoviridae: Measles Virus pt. 2 □ CD – discovered or have a function in the immune system □ Slightly different strategy from the rhabdo virus  P is a co­factor for L protein □ P ­ cofactor  Rhabdoviridae & Paramyxoviridae □ Nuclocapside proteins – first to transcribe 
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