EESB16H3 Lecture Notes - Genetically Modified Organism, Nucleic Acid Hybridization, Transgene

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Recombinant biotechnology: how to move genes between organisms. Recombinant dna transfers genes from one species to factory organisms to increase production of rare proteins/enzymes: manipulation of genetic content of organisms to alter their properties, may include the introduction of transgenes from other organisms. Transgene: an exogenous gene introduced to genome of another organisms: steps for rdna: Biodiversity search for desirable traits find genetic determinants of such traits . Extract dna from organisms with desirable traits isolate genes of interest aka target genes (tg) clone genes into bacteria/yeast etc. for mass production, alteration and re-association with other genetic elements. Clone genes are put into bacteria/yeast for mass production. In bacteria, alteration and reassociation of genetic sequence occurs to insert the introduced genetic elements: they can be introduced to plant tissues. Plants are grown and selected for desirable genetically modified plants. These plants are used as parents for future hybrids.

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