EESB16H3 Lecture Notes - Lecture 8: Nucleic Acid Hybridization, Sticky And Blunt Ends, Selective Breeding
Document Summary
Classical biotechnology: making of wine, beer, cheese, bread, soy sauce, production of antibiotics, selective breeding of crops/livestock, exploitation of biodegradative bacteria. General flow: biodiversity, search for desirable traits, find genetic determinants of such traits, extract dna from organisms with desirable traits. Isolate genes of interest target genes (tg: clone genes into bacteria/yeast for mass production, alteration, and association with other genetic elements a) Introduction to plant tissues: grow and select for desirable gm plants (maintain gm plant cell line, use as parental sources for hybrids, test and market good hybrids b) Introduction to animal germ tissues: hatch or implant and gestate, raise, select for desirable properties, breed, select offspring with tg in germ cells (egg, sperm, clone, or breed adult gm organisms for market. Molecular or gene cloning: cloning of dna (genes/virus/regulatory elements) for lab production. Cellular cloning: derive a cell line from single cells, bacteria, yeast, tissues, stem cells. Organism cloning: derive multicellular adult from embryonic or adult cells.