BCH210H1 Lecture Notes - Lecture 18: Nicotinamide Adenine Dinucleotide, Carnitine O-Palmitoyltransferase, Alanine Transaminase

• School: UTSG
• Department: Biochemical Engineering and Applied Chemistry
• Course: BCH210H1
• Professor: Reinhart Reithmeier

Document Summary

Enzyme present in cells : selective catalysis, highly specific for its substrate, can be stereospecific, catalyze specific reaction. Hydrolase: largest class of enzymes, use water to lyse (h-oh, pyrophosphatase (ppi to pi & pi), proteases, lipases, nucleases, esterases, subtilisin, rnase (proteases) Lyase/synthase: create = or ring (after addition/removal of group, not using redox, not using water (hydrolysis, pyruvate decarboxylase (lose coo-, will form co2 to stabilize, synthase if other direction more important. Bis = same group on different carbons (1,3-bpg phosphoglyerate mutase 2,3-bpg) Join molecules, use atp (needs energy, not using atp like kinase: make fatty acyl + coa, produce adp + pi (use atp) Characteristics that define enzymes (1) catalytic power (2) specificity (3) regulation (4) enzyme nomenclature (5) coenzymes & cofactors. Catalytic power: catalytic power of enzyme = (catalyzed rate) / (uncatalyzed rate, all enzymes have catalytic rate.