BCH311H1 Lecture Notes - Lecture 4: Origin Recognition Complex, High Fidelity, Atp Hydrolysis
26 Aug 2018
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Dna polymerase: kornberg, lehman, bessman, and simms, 1956. It always seemed to me that a biochemist devoted to enzymes could, if persistent, reconstitute any metabolic event in the test tube as well as the cell does it. Thought that thymidine was not the direct precursor for the dna. It was part of the same polypeptide as dna polymerase one (as it has two active sites: at the nuclease site it has a endo and exo sites - the exo from the end (3"- Dna polymerase i does not replicate the e. coli chromosome. John cairns + paula delucia isolated a mutant e. coli with no dna pol i activity. Can you think of 2 technologies that were enabled by understanding how dna polymerases work: pcr, was discovered by scientists that succeeded this discovery, sanger sequencing, ngs mostly rely on this.
