BIO120H1 Lecture Notes - Lovebird, Lysis, Exponential Growth

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2 Feb 2013
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BIO120H1 Full Course Notes
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BIO120H1 Full Course Notes
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Lecture outline: pcr, forensic applications, isolating a gene using pcr with degenerate primers, cdna synthesis. A no: needs to be group 2. Consensus sequence signaling at the beginnings and the ends of introns are exactly the same in types of splicing. No: exon size tends to be more uniform than intron size which helps the cell to choose the correct splice sites. The binding of the splicesome components such as u1 and sr onto the nascent bead as it emerges from the rna polymerase tend to increase splice error. This technique was a revolution in about the late 80"s or so. Pcr is an extremely sensitive & fast technique to make many copies of a given sequence that you"re interested in. Polymerase chain reaction (pcr) came up by kary mullis. Repeat the 3 steps many times approximately 25 or 30 times. Tac is a type of dna polymerase that was isolated from a bacterium which grows in very hot springs.

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