BIO120H1 Lecture Notes - Restriction Enzyme, Photodetector, Endonuclease
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BIO120H1 Full Course Notes
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Today: restriction endonucleases, electrophoresis serial dilutions can also be used to determine cell concentrations! from: brock biology of. Bacterial growth: requires nutrients and specific physical conditions, determining growth stage is important in molecular biology e. g. , for nucleic acid isolations, need to know how bacteria grow, and how growth can be measured. Bacterial growth = cell division: aka: binary fission, cells divide into 2 daughter cells, # of cells double each generation. Exponential growth cell number versus time: standard and semi-log plots exponential growth shown by linearity of semi-log plot figures from: brock biology of microorganisms, 11th edition. chapter 6. Bacterial growth phases refers to populations, not individuals! from: brock biology of microorganisms, 11th edition. Nucleases are enzymes that cleave phosphodiester bonds between nucleotides of nucleic acids. Exonucleases: cut nucleic acids from the ends of the molecule. Endonucleases: cut nucleic acids internally restriction endonucleases are also called restriction enzymes. Enzymes that cut dsdna at specific dna sequences they recognize.
