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15 Mar 2011
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LECTURE 17 โ€“ Extracellular matrix and cell motility
๎€Human fibroblast cells grown in tissue culture
Cell-Matrix adhesion
Cell to cell attachments โ€“ 3 main
๎€Cell cell anchoring junction โ€“ adhering junction โ€“ actually holds cells together
๎€Tight junction โ€“ so things cannot pass between cells
๎€Channel forming gap junctions โ€“ allow small materials pass from cell to cell
๎€Focal adhesion โ€“ below the left hand bottom paragraph
oContact btw basal surface and extracellular matrix
oImportant for cell migration
ECM โ€“ drawing of basal surface of cell
๎€Green are transmembrane proteins, integrins
๎€Complex network of proteins and sugars
๎€Mesh is extracellular matrix
By fibroblastsโ€
๎€Fibroblast secretes most of extracellular matrix
๎€Epithelial cell layer โ€“ apical at top
๎€Basal lamina type of ECM
๎€Basal faces ECM
๎€Yellow line around capillary
๎€Fibroblast secrete most of the ECM
Triangular cell โ€“ looking at fibroblast
Two major kind of ECM
๎€(Basal lamina)โ€
๎€(connective tissue)โ€
๎€Muscle contract โ€“ pulls on basal lamina, which pulls on connective tissue, which
pulls on bone
๎€Capillary โ€“ endothelial cells attached to basal lamina
oKidney has lining of tubules โ€“ no tight junctions between cells โ€“ allows
filtering function SO blood cells not found in urine
ECM
๎€Structure and regulates behaviour
๎€Tensile strength; resistance to compression
Reinforced concrete
๎€Metal beams or wires
๎€Together v strong structure, having tensile strength AND resistance to
compression
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๎€If separated
oChain-link fence โ€“ easily compressed BUT has resistance to knocking and
stretching forces (AKA tensile strength)
oCement โ€“ highly resistant to compression but little resistance to knocking
forces
๎€Like reinforced concrete, one for strength one for compression
ECM important becauseโ€ฆ
๎€Defects in ECM lead to human diseases
๎€Collagen fibres not assemble properly โ€“ scurvy
๎€Ehlers-Danlos syndrome โ€“ v. stretchy skin
๎€All listed are collagen defects โ€“ how collagen offers tensile strength to tissue
Two main classes of ECM macromolecules
๎€GAGs made of sugar
๎€Fibrous proteins โ€“ 4 kinds
GAGs
๎€Amino group included
๎€Disaccharide unite โ€“ two sugars
๎€Know N-acetylglucosamine + glucurojnic acid example
๎€Chain of sugars can get extremely long******
oImportant for function
oActing as filler โ€“ so should be very long
๎€OH group in sugar โ€“ used in covalent linkage btw sugars โ€“ once linked, becomes
O involved in covalent bond
๎€Negative charge ****
oSulphate groups, carboxy groups
oNegative charges repel each other
oLong molecule repelling itself ERGO straighten out
๎€Negative charges will attract cations ***
oSalty molecule will attract water***
oWater molecules inflate this molecule so this fills a large space ERGO
spongy spacefilling stuff between cells
GAGs
๎€Because of attached sulphate and carboxyl groupsโ€
๎€Making proteoglycansโ€
oCovalently attached to proteins
GAGs
๎€Four main groups of GAGs
๎€Hylauronan โ€“ not linked to proteins
๎€The bottom three are linked to proteins
๎€ KNOW THESE NAMES
๎€Cube with single Hyluronan molecule swelled into large area
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Document Summary

Lecture 17 extracellular matrix and cell motility. human fibroblast cells grown in tissue culture. Tight junction so things cannot pass between cells. cell cell anchoring junction adhering junction actually holds cells together. channel forming gap junctions allow small materials pass from cell to cell. Focal adhesion below the left hand bottom paragraph: contact btw basal surface and extracellular matrix, important for cell migration. Ecm drawing of basal surface of cell. epithelial cell layer apical at top. Two major kind of ecm (basal lamina) (connective tissue) . muscle contract pulls on basal lamina, which pulls on connective tissue, which pulls on bone. capillary endothelial cells attached to basal lamina: kidney has lining of tubules no tight junctions between cells allows filtering function so blood cells not found in urine. Together v strong structure, having tensile strength and resistance to compression www. notesolution. com.

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