BIO130H1 Lecture 3: Coleman Lecture Three

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13 Mar 2012
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BIO130H1 Full Course Notes
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Molecular interactions: interactions between individual molecules usually mediated by non- covalent attractions. Individually, very weak forces can sum to generate strong binding between molecules. Rna synthesis (transcription) protein synthesis (translation) + dna synthesized from deoxyribonucleoside triphosphates known as dntp"s. + rna synthesized from ribonucleic triphosphates ntp"s. + one strand is 5" 3"; its partner is 3" 5" + important for dna replication & rna synthesis. + heating denatures double-stranded dna by disrupting h-bonds between bases. + temperature at which dna denatures is called tm & it varies between species. + denaturation of dna is a reversible process. Amino acid side-chain (r-groups) is variable and determines types of amino acid. + purpose: amplify dna make copies of that dna sequence when denature by heating, will unwind into two separate dna strands. Dna (make complimentary strand) to the two separated parent strands of dna. When cool dna back down (renatuing), get twice as much as dna.

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