BIO130H1 Lecture 5: Lecture 5 Hour 1

• School: UTSG
• Department: Biology
• Course: BIO130H1
• Professor: Jane Mitchell

Document Summary

Feb. 2/12: newly synthesized strand is synthesized from 5"-3". The 5" of the complementary strand attaches to the 3" of the parent. Origin identification experiment (circular genome, bacteria: use ars (autonomously replicating sequences) in a yeast cell, ars functions as an origin of replication, compare two yeast cells with a common gene (ex. Insert possible ars segment in one and random dna segment in the other: replication and common gene expression should be greater in ars induced cell. Pull double stranded circular genome apart: replication occurs at replications forks, bi-directional growth from one starting point (left and right) Forks are points where parental strands are pulled apart. Replication at forks: obeys two rules, dna strands are anti-parallel, newly synthesized end adds on to 3" end of parent strand. Parental strands divided into leading and lagging template. Leading template runs from 3"-5" (5" at replication fork) Leading strand synthesizes as a continuous strand from its 5"-3".