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Second Half Lecture 1 Part 2 (lec2)

Course Code
Jane Mitchell

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Lecture 2 Mar. 1/12
Cellular Form and Function
Bilayer division
Cytosolic face (leaflet): usually faces the cytosol, not always
Exoplasmic face (leaflet): faces inside the lumens of organelles or outside of the cell
Through organelle formation/transformation, face types stay constant
Asymmetrical use of Bilayer
Glycolipids synthesized on luminal (exoplasmic) face
Phospholipids bind cytosolic proteins
Transmembrane proteins
Pass through entire lipid bilayer
Hydrophilic domains when in contact with aqueous solution
Hydrophobic domains when in contact with non-polar molecules
Act as receptors (for signalling) and as ion/particle channels
Lipid anchored membrane proteins
Amphiphilic alpha-helix anchors itself in cytosolic face of bilayer
Hydrophobic regions attach to non-polar tails
Hydrophilic regions bind to various proteins, anchor directs proteins to cytosolic face
Peripheral membrane proteins
Does not insert itself into bilayer
Binds to other proteins on either face by non-covalent interactions
Extraction of proteins from membrane
Peripheral membrane proteins use gentle extraction, lipid bilayer conserved
Integral membrane proteins require detergents to destroy bilayer for extraction
Detergent: small amphiphilic molecules that destroy the bilayer
Fluorescence recovery after photobleaching (FRAP)
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