BIO130H1 Lecture : Second Half Lecture 1 Part 2 (lec2)
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BIO130H1 Full Course Notes
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Bilayer division: cytosolic face (leaflet): usually faces the cytosol, not always. Exoplasmic face (leaflet): faces inside the lumens of organelles or outside of the cell. Asymmetrical use of bilayer: glycolipids synthesized on luminal (exoplasmic) face. Pass through entire lipid bilayer: amphiphilic, hydrophilic domains when in contact with aqueous solution, hydrophobic domains when in contact with non-polar molecules, act as receptors (for signalling) and as ion/particle channels. Lipid anchored membrane proteins: amphiphilic alpha-helix anchors itself in cytosolic face of bilayer, hydrophobic regions attach to non-polar tails, hydrophilic regions bind to various proteins, anchor directs proteins to cytosolic face. Peripheral membrane proteins: does not insert itself into bilayer, binds to other proteins on either face by non-covalent interactions. Peripheral membrane proteins use gentle extraction, lipid bilayer conserved. Integral membrane proteins require detergents to destroy bilayer for extraction: detergent: small amphiphilic molecules that destroy the bilayer.