BIO130H1 Lecture Notes - Lecture 10: John Wiley & Sons, Rna Polymerase Ii, Transcription Factor Ii A
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BIO130H1 Full Course Notes
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Bio130 lecture 10 - transcription part ii. Special mushroom: aminita phalloides: relevant characteristics to lecture, produces a toxin that binds eukaryotic rna polymerase ii and binds right in the active site where the dna-rna hybrid is made, this will not be tested. Eukaryotes: transcription in prokaryotes, structure of a gene, rna polymerases, transcription of protein-coding genes, rna processing. Rnap enters processive mode and sigma factor releases. Termination occurs using two mechanism: rho dependent: protein called rho that is like a ring, it travels along the mrna and dislodge mrna from the rna polymerase. Much less common than rho independent: the important one is rho independent. Termination sequence is gc-rich area followed by at-rich area. Creates hairpin loop with gc loop and then au section. Once the polymerase hits the loop, it will be pulled off. The h-bonding between as and ts is weaker than g-c. The initial steps of rna synthesis are relatively inefficient.
