BIO130H1 Lecture Notes - Lecture 6: Condensin, Barr Body, Heterochromatin
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BIO130H1 Full Course Notes
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Week 3 lectures (and a bit of week 4) today Lecture outline: genomes (slides from last week, bacterial dna packaging (slides from last week, introduction to eukaryotic dna packaging (slides from last week, introduction to eukaryotic chromosomes, nucleosomes, chromatin structure. Readings (from last week): 411-414; 400-406 (skip human perspectives and focus on complexity of eukaryotic genomes section) Getting the eukaryotic genome packed into cells: the chromosome solution. Karyotype artificial array of paired chromosomes in numerical order diagnostic aid. A technique fish (fluorescent in situ hybridization): uses h bonding between complementary base pairs. Target dna, each chromosomes have specific and unique sequences. Design probe with the sequence complementary to the target, then label. Probe with fluorescent, when it anneals, you"re able to see the signal. Denature the chromosome, the heat loosen up the chromosome. Slowly cool the solution so it anneals. Each chromosome contains a single, long linear dna molecule and associated proteins called chromatin.
