BIO130H1 Lecture Notes - Lecture 9: Polypyrimidine Tract, Autocatalysis, Ribozyme
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BIO130H1 Full Course Notes
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In rnap ii only the carboxy terminal domain of the largest subunit has a stretch of 7 amino acids that is repeated multiple times. Phosphorylation of serine 5 on the ctd domain. Ctd allow the rnap get into its processive mode, also attracts other factors. As nascent rna is made, a 5" cap is put on it right away. Chromatin remodeling complex: help the transcription machinery get through chromatin. Histone modification complex: help polymerase get through. Enhancer sequence: help promoter in terms of how much rna is transcribed. Ctd induces a conformational change, allow it to get into the processive mode. Adding phosphate groups on s (ser) located on the ctd. Phosphorylation of c terminal tail of rna. Eg. 5" capping enzyme, spliceosome, protein for cleavage, elongation factors etc. Without it, the cell tend to degrade mrna. Important for making sure the mrna is transported out of the nucleus. Rna triphosphatase: clip off the outermost phosphate group on the 5"end.
