BIO230H1 Lecture Notes - Lecture 4: Dna-Binding Domain, Rna Polymerase Ii, Eukaryotic Transcription
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BIO230H1 Full Course Notes
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Lecture 4 - eukaryotic gene regulation: eukaryotic transcriptional activation, eukaryotic transcriptional repression. Recall that dna is transcribed into rna by the enzyme rna polymerase. Produce rna molecule complementary to one strand of dna. Rnap ii transcribes all protein coding genes. Different rnas transcribed by different rna polymerases in eukaryotes. Prokaryotes have a single type of rna polymerase. Only a sigma factor required to initiate. Transcription initiation in eukaryotes requires many proteins: general transcription factors. Help position rna polymerase at eukaryotic promoters contain tata box. Tfiid: tata binding domain - find tata box. Recruits rest of the complex initiate. Helicase activity: split apart dna helix and expose the strand. Kinase activity: phosphorylates the c- terminal end of rnap ability for rnap to initiate transcription. Required by nearly all promoters used by. Rna polymerase ii transcribes protein coding genes. Tfiid, tfiib, tfiif,(prokaryotes only need one sigma factor ) Each gene has its own promoter and own rna molecule.
