BIO230H1 Lecture Notes - Lecture 11: Programmed Cell Death, Death Effector Domain, Card Domain

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BIO230H1 Full Course Notes
BIO230H1 Full Course Notes
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W/ acute injury cell swells & bursts. Definition: programmed cell death: shrinks the cells, collapses cytoskeleton, fragments the dna, signals the macrophages to engulf the cell. Active form- they"re cysteine proteases that cleave target proteins at specific aspartic acid residues. Attached to prodomains- keeps it in inactive conformation. 2 inactive caspases come close in proximity. Phosphorylates each other removes prodomains active caspase. Caspase amplification cascade: initiator caspase (1 molecule of active caspase, executioner caspases activated, leads to cleavage of proteins & dna. Activating caspase cascades: extrinsic signals, intrinsic signals. Cytochrome c release from damaged mitochondria: inactive apaf1 always present in the cell, cytochrome c release, cytochrome c- apaf1 binding conformational change exposes. Card domain: card domain forms complex w/ other card domains apoptosome, apoptosome recruits procaspases into a close proximity cleaving active caspases, caspase cascade apoptosis. Protect from accidents: decoy receptors (competitive inhibition, decoy initiator caspase (competitive inhibition, block apoptotic machinery (physical changes)